2. Metabolic Enzyme/Protease
  3. Glucosidase
  4. Fagomine

Fagomine  (Synonyms: 荞麦碱; D-Fagomine)

目录号: HY-13005 纯度: 98.97%
COA 产品使用指南

摩尔计算器

Fagomine 是一种温和的糖苷酶 (glycosidase) 抑制剂，Fagomine 作用于 Amyloglucosidase (A.niger)，β-Glucosidase (bovine) 和 Isomaltase (yeast)，Ki 分别为 4.8 μM，39 μM 和 70 μM。

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Fagomine Chemical Structure

Fagomine Chemical Structure

CAS No. : 53185-12-9

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥4950
In-stock
1 mg ￥2045
In-stock
5 mg ￥4500
In-stock
10 mg 现货 询价
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Fagomine 相关抗体

Top Publications Citing Use of Products

    Fagomine purchased from MCE. Usage Cited in: J Agric Food Chem. 2018 Mar 21;66(11):2758-2764.  [Abstract]

    Effect of D-fagomine on the expression of phosphorylated LKB1, AMPK, SIRT1, and PGC-1α in HUVECs. Cultured HUVECs are treated with D-fagomine (0.1, 1, or 10 μM) for 24 h. Cell lysates are prepared and subjected to Western blotting analyses.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Fagomine is a mild glycosidase inhibitor. The Ki of the iminosugar Fagomine is 4.8 μM, 39 μM, and 70 μM for Amyloglucosidase (A.niger), β-Glucosidase (bovine), and Isomaltase (yeast), respectively.

    IC50 & Target

    Glycosidase[1]
    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    RAW IC50
    > 10 μM
    Compound: 28
    Inhibition of glucosylceramide synthase in mouse RAW cells preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence scanner
    Inhibition of glucosylceramide synthase in mouse RAW cells preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence scanner
    		[PMID: 24900342]
    体外研究
    (In Vitro)

    Fagomine (D-fagomine) 是一种亚氨基糖，可在体外选择性凝集肠杆菌。Fagomine 选择性地凝集流苏状肠杆菌 (例如，E.coli) 并抑制它们对肠粘膜的粘附；其原因可能与其与凝集素结合糖类 (如甘露糖) 的结构相似性有关。Fagomine 能够改变高脂肪高蔗糖饮食 (HFHS) 对肠杆菌和大肠杆菌比例的影响[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Fagomine 相关抗体:
    体内研究
    (In Vivo)

    Fagomine (D-fagomine) 是一种天然亚氨基糖，可抵消高能量密度饮食对体重、空腹血糖水平和肠道肠杆菌的短期影响[3]。与标准组相比，喂食高脂肪高蔗糖饮食 (HFHS) 和 Fagomine (D-fagomine) 的大鼠体重增加 (15.3%) 明显低于喂食 HFHS 的大鼠 (20.9%)[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    147.17

    Formula

    C6H13NO3
    CAS 号
    性状

    固体

    颜色

    White to khaki

    中文名称

    荞麦碱；桑多酚；桑叶生物碱
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : ≥ 36 mg/mL (244.62 mM)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.7949 mL 33.9743 mL 67.9486 mL
    5 mM 1.3590 mL 6.7949 mL 13.5897 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (679.49 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 98.97%

    COA (191 KB) HNMR (246 KB) RP-HPLC (213 KB)

    产品使用指南 (1538 KB)
    参考文献
    Kinase Assay
    [1]

    Lysosomal enzyme activities in cell lysates are determined. Briefly, cells are scraped in ice-cold 0.1% Triton X-100 in water. After centrifugation (6000 rpm for 15 min at 4°C) to remove insoluble materials, protein concentrations are determined using Protein Assay Rapid Kit. The lysates are incubated at 37°C with the corresponding 4-methylumbelliferyl β-D-glycopyranoside solution in 0.1 M citrate buffer (pH 4). The liberated 4-methylumbelliferone is measured with a fluorescence plate reader (excitation 340 nm; emission 460 nm). For enzyme inhibition assay, cell lysates from normal skin fibroblasts are mixed with the 4-methylumbelliferyl β-D-glycopyranoside substrates in the absence or presence of increasing concentrations of Fagomine[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [1]

    Human skin fibroblasts from a healthy and three Gaucher disease patients (with N188S/G183W, V230G/R296X and L444P/L444P mutations) are maintained in our laboratory with DMEM supplemented with 10% FBS as the culture medium. For enzyme activity enhancement assay, cells are cultured in the presence of different concentrations of Fagomine or DMSO alone (as a control) for 5 days and harvested by scraping. Cytotoxicity of Fagomine is monitored by measuring the lactate dehydrogenase activities in the cultured supernatants[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Rats[3]
    Sprague-Dawley rats (male, 22 weeks old) are randomly assigned to one of the three dietary groups: the control group, fed a standard diet (STD); a group fed HFHS (modified high-fat high-sucrose diet); and a group fed HFHS supplemented with 0.065% Fagomine (HFHS+FG). The percentage of Fagomine is adjusted so that its ratio to sucrose is 2 mg/g, as defined before from the results of post-prandial tests.The modified diets are processed. Feed consumption is monitored every day throughout the experiment and body weight is measured before and at the end of the nutritional intervention. All animal manipulations are carried out in the morning to minimize the effects of circadian rhythms.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Fagomine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.7949 mL 33.9743 mL 67.9486 mL 169.8716 mL
    5 mM 1.3590 mL 6.7949 mL 13.5897 mL 33.9743 mL
    10 mM 0.6795 mL 3.3974 mL 6.7949 mL 16.9872 mL
    15 mM 0.4530 mL 2.2650 mL 4.5299 mL 11.3248 mL
    20 mM 0.3397 mL 1.6987 mL 3.3974 mL 8.4936 mL
    25 mM 0.2718 mL 1.3590 mL 2.7179 mL 6.7949 mL
    30 mM 0.2265 mL 1.1325 mL 2.2650 mL 5.6624 mL
    40 mM 0.1699 mL 0.8494 mL 1.6987 mL 4.2468 mL
    50 mM 0.1359 mL 0.6795 mL 1.3590 mL 3.3974 mL
    60 mM 0.1132 mL 0.5662 mL 1.1325 mL 2.8312 mL
    80 mM 0.0849 mL 0.4247 mL 0.8494 mL 2.1234 mL
    100 mM 0.0679 mL 0.3397 mL 0.6795 mL 1.6987 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fagomine53185-12-9D-Fagomine荞麦碱桑多酚桑叶生物碱GlucosidaseInhibitorinhibitorinhibit

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